For this procedure an oocyte (egg) is collected from the donor mare’s follicle prior to ovulation and cultured until it is mature. The egg is then fertilized in the laboratory by ICSI (Intracytoplasmic Sperm Injection). In this procedure, a single sperm cell is selected and micro-injected into the matured egg.
Once the egg divides, usually within 24 hours following fertilization, it is cultured to the blastocyst stage before transfer to a recipient mare. Since only a single sperm cell is selected and injected, ICSI can utilize very small quantities of frozen semen.